Search results for " saline"

showing 10 items of 74 documents

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Use of Gibberellic Acid to Increase the Salt Tolerance of Leaf Lettuce and Rocket Grown in a Floating System

2020

Hydroponics need water of good quality to prepare a balanced nutrient solution that could allow plants to reach their maximum yield potential. The rising difficulties in finding water with good quality have led to the compelling necessity of identifying sustainable ways to use saline water, limiting its negative effect on crop yield and quality. The exogenous supplementation of plant growth regulators, such as gibberellic acid (GA3), can be effective in increasing plant growth and vigor, thus helping plants to better cope with salt stress. The aim of this study was to evaluate the feasibility to increase the salt tolerance of leaf lettuce and rocket grown in a floating system by adding GA3 …

0106 biological sciencesStomatal conductancesaline waterLactuca sativa L. var. CrispaBiomassSettore AGR/04 - Orticoltura E FloricolturaEruca sativa L.01 natural scienceslcsh:Agriculturechemistry.chemical_compoundGA3nitrateleafy vegetablesGibberellic acidCrop yieldfungilcsh:Sfood and beverageshydroponics04 agricultural and veterinary sciencesHydroponicsAscorbic acidSaline waterSalinityHorticulturechemistry040103 agronomy & agriculture0401 agriculture forestry and fisheriesascorbic acidAgronomy and Crop ScienceAscorbic acid Eruca sativa L GA3 Hydroponics Lactuca sativa L. var. Crispa Leafy vegetables Nitrate Saline water010606 plant biology & botanyAgronomy
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The role of extracellular polymeric substances on aerobic granulation with stepwise increase of salinity

2018

Abstract A granular sequencing batch reactor (GSBR) worked for 164 days to study the effect of salinity on aerobic granulation. The feeding had an organic loading rate (OLR) of 1.6 kg COD⋅m −3 ⋅d −1 and a gradual increase of salinity (from 0.30 to 38 g NaCl − ⋅L −1 ) to promote a biological salt-adaptation. First aggregates (average diameter ≈ 0.4 mm) appeared after 14 days. Extracellular polymeric substances (EPSs) analyses revealed that proteins were mainly higher than polysaccharides, and microorganisms metabolized EPSs as additional carbon source, mostly in feast phase, to face the energy demand for salinity adaptation. No significant worsening of organic matter removal was observed. Th…

0208 environmental biotechnologyHydrophobicitySequencing batch reactorFiltration and Separation02 engineering and technology010501 environmental sciences01 natural sciencesAcclimatizationNutrients removalAnalytical ChemistryAerobic granular sludge; EPS; Extracellular polymeric substances; Hydrophobicity; Nutrients removal; Saline wastewater; Analytical Chemistry; Filtration and SeparationExtracellular polymeric substanceExtracellular polymeric substanceOrganic matterFood science0105 earth and related environmental scienceschemistry.chemical_classificationSettore ICAR/03 - Ingegneria Sanitaria-Ambientale020801 environmental engineeringSalinitychemistryAerobic granular sludgeAerobic granulationNitrificationEPSSaline wastewaterAnaerobic exercise
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Oil-degrading bacteria from a membrane bioreactor (BF-MBR) system for treatment of saline oily waste: Isolation, identification and characterization …

2016

A collection of forty-two (42) strains was obtained during microbiological screening of a Membrane Bioreactor (MBR) system developed for the treatment of saline oily waste originated from marine transportation. The diversity of the bacterial collection was analyzed by amplification and sequencing of the 16S rRNA gene. Taxonomic analysis showed high level of identity with recognized sequences of seven (7) distinct bacterial genera (Alcanivorax, Erythrobacter, Marinobacter, Microbacterium, Muricauda, Rhodococcus and Rheinheimera). The biotechnological potential of the isolates was screened considering an important factor such as the biosurfactant production. In particular fourteen (14) biosur…

0301 basic medicine030106 microbiologyMicrobacteriumOil pollution010501 environmental sciencesMembrane bioreactor01 natural sciencesMicrobiologyMicrobiologyBiomaterials03 medical and health sciencesAlcanivoraxOil-degrading bacteriaAlcanivorax Membrane bioreactor (MBR) system Oil pollution Oil-degrading bacteria Saline oily wasteWaste Management and DisposalMembrane bioreactor (MBR) system0105 earth and related environmental sciencesbiologyMarinobacter16S ribosomal RNAbiology.organism_classificationIsolation (microbiology)Saline oily wasteAlcanivoraxRhodococcusBacteria
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THE ENVIROMENTAL PROBLEMS OF THE PIANA DEL SIGNORE (GELA, SICILY) FOR A CORRECT AGRONOMIC, CULTURAL AND LANDSCAPE UPGRADING AND ENHANCEMENT OF THE AR…

2017

In Sicily it is witnessing an environmental degradation that can be conducted to the human activity and the already known process of desertification. The present study had as object the c/da Piana del Signore in plain of Gela being an area characterized by an extreme aridity and salinity. Through consultation and study of the various researches realized on the area, the various environmental and territorial characteristics outlined, highlighting the issues and possible improvements. The objective was to outline the damages caused by man proposing possible routes for a correct requalification of the area in order to enhance a landscape of great agronomic and naturalistic interest. In fact to…

0301 basic medicineGela; saline soils; aridity; pollution; upgrading04 agricultural and veterinary sciencesGelasaline soils03 medical and health sciencesGela saline soils aridity pollution upgrading030104 developmental biologylcsh:Environmental pollutionariditySettore AGR/14 - Pedologialcsh:TD172-193.5040103 agronomy & agriculturepollution0401 agriculture forestry and fisheriesupgrading
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Thermomineral waters of Greece: geochemical characterization

2020

75 °C). In terms of pH most results vary from 5.5 to 823 °C) ii) warm (23 40 °C) iii) thermal (40 75 °C) and iv) hyperthermal (&gtfew springs show either very low pH (&lt10) proposing serpentinization processes. Regarding TDS concentrations collected waters can be subdivided into low salinity (up to 1.5 g/L) brackish (up to 20 g/L) and saline (up to 43 g/L). The medium high salinities can be justified by mixing with sea water and/or strong waterrock interaction processes. Isotope composition of O and H ranges from 12.7 to +2.7 ‰ SMOW and from 91 to +12 ‰ SMOW respectively and is generally comprised between the Global Meteoric Water Line and the East Mediterranean Meteoric Water Line. Only few water samples show a positive shift for δ18O possibly related to high temperature waterrock interaction processes. Carbon dioxide (18 997000 μmol/mol) or N2 (1100 989000 μmol/mol) or CH4 (&ltMany geothermal areas of Greece are located in regions affected by Miocene or Quaternary volcanism and in continental basins characterised by elevated heat flow. Moreover the majority of them is found along the coast as well as in islands of the Aegean Sea and thus thermal water is often brackish to saline due to marine intrusion into costal aquifer. In the present study almost 300 thermal and cold mineral water samples were collected along the Hellenic territory with their physicochemical parameters (temperature pH electrical conductivity and Eh) and the amount of bicarbonates (titration with 0.1N HCl) being determined in situ. Additionally gases found either in free or dissolved phase were sampled. Both water and gas samples were analysed at the INGVPa laboratories for major ions (Ion Chromatography) silica (Inductively Coupled Plasma Optical Emission Spectrometry) chemical composition of free and dissolved gases (Gas Chromatography) water isotopes (O and H) and carbon and helium isotopes of free and dissolved gases (Mass Spectrometry). The temperature of the investigated waters ranges from 6.5 to 98°C pH from 1.96 to 11.98 whilst Total Dissolved Solids (TDS) from 0.06 to 43 g/L. Based on the temperature parameter waters can be divided into four groups: i) cold (&lt0.5 913000 μmol/mol) are the prevailing gas species found in the studied sites. The δ13CCO2 values ranged from 20.1 to +8.5 ‰ whilst the isotope ratio of He from 0.21 to 6.71 R/RA.4) suggesting interaction with H2Srich gases or very high pH values (&gtSettore GEO/08 - Geochimica E Vulcanologia
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Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo

2021

Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly due to the inhibitor specificity, toxicity, and drug interactions. Here, we reported that three polyoxypregnanes (POPs) as the most abundant constituents of Marsdenia tenacissima (M. tenacissima) were novel ABCB1-modulatory pro-drugs, which underwent intestinal microbiota-mediated biotransformation in vivo to generate active metabolites. The metabolites at non-toxic concentrations restored chemosensitivity in ABCB1-overexpressing cancer cells v…

ABCC1 ATP binding cassette subfamily C member 1IC50 half maximal inhibitory concentrationMultidrug resistancePharmacologyNADPH reduced nicotinamide adenine dinucleotide phosphateF bioavailabilitychemistry.chemical_compoundPCR polymerase chain reaction0302 clinical medicineMDR multidrug resistanceECL electrochemiluminescencet1/2 elimination half-lifeLC–MS liquid chromatography coupled with mass spectrometryN.D. not detectedGeneral Pharmacology Toxicology and PharmaceuticsBBB blood–brain barriermedia_commonATF3 activating transcription factor 30303 health sciencesChemistryABC ATP-binding cassetteNMPA National Medical Products AdministrationPXR pregnane X receptorSDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresisHBSS Hankʹs balanced salt solutionABCB1Combination chemotherapyProdrugMarsdenia tenacissimaCmax peak concentrationPaclitaxelGAPDH glyceraldehyde-3-phosphate dehydrogenase030220 oncology & carcinogenesisBHI brain heart infusionOriginal ArticleAUC0–∞ area under plasma concentration vs. time curveMRT mean residence timeDrugmedia_common.quotation_subjectRM1-950Vd volume of distributionABCB1 ATP binding cassette subfamily B member 1UIC-2 mouse monoclonal ABCB1 antibodyABCG2 ATP binding cassette subfamily G member 2Combination chemotherapyCYP cytochrome P450 isozymePI propidium iodideTEER transepithelial electrical resistance03 medical and health sciencesPBS phosphate buffer salineFBS fetal bovine serumDox doxorubicinIn vivoPOP polyoxypregnanemedicine030304 developmental biologyEVOM epithelial tissue voltohmmeterTmax time for peak concentrationCancerLBE lowest binding energyPE phycoerythrinmedicine.diseaseMultiple drug resistancePolyoxypregnanePapp apparent permeabilityN.A. not applicableCancer cellH&E hematoxylin and eosinMDR1a multidrug resistance protein 1aTherapeutics. PharmacologyqPCR quantitative PCRM. tenacissima Marsdenia tenacissimaCL clearanceSD standard derivationActa Pharmaceutica Sinica B
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Clinical Trial: High-dose furosemide plus small-volume hypertonic saline solutions vs. repeated paracentesis as treatment of refractory ascites.

2009

Summary Background  In patients with cirrhosis, ascites is defined as refractory when it cannot be mobilized or recurs early in standard diuretic therapy. Aim  To compare the safety and efficacy of intravenous high-dose furosemide + hypertonic saline solutions (HSS) with repeated paracentesis in patients with cirrhosis and refractory ascites. Patients and methods  Eighty-four subjects (59/25 M/F) with cirrhosis, mostly of viral aetiology, admitted for refractory ascites, were randomly assigned to receive furosemide (250–1000 mg/bid i.v.) plus HSS (150 mL H2O with NaCl 1.4–4.6% or 239–187 mEq/L) (60 patients, Group A) or to repeated paracentesis and a standard diuretic schedule (24 patients,…

AdultLiver CirrhosisMalemedicine.medical_specialtyCirrhosismedicine.medical_treatmentPilot ProjectsRefractoryFurosemideAscitesmedicineParacentesishypertonic saline solutionHumansParacentesisPharmacology (medical)DiureticsAgedAged 80 and overSaline Solution HypertonicRCT; hypertonic saline solution; ascitesHepatologymedicine.diagnostic_testDose-Response Relationship Drugbusiness.industryGastroenterologyFurosemideAscitesMiddle Agedmedicine.diseaseHypertonic salineSurgeryTreatment OutcomeEffusionAnesthesiaFemaleDiureticmedicine.symptombusinessRCTmedicine.drugAlimentary pharmacologytherapeutics
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Impact of hypertonic saline nebulisation combined with oscillatory positive expiratory pressure on sputum expectoration and related symptoms in cysti…

2018

Abstract Objective To evaluate the impact of combining nebulised hyaluronic acid plus hypertonic saline (HA + HS) with oscillatory positive expiratory pressure (oscillatory-PEP) on sputum expectoration and related symptoms in adults with cystic fibrosis (CF). Design Randomised crossover trial. Setting Seven centres. Participants Twenty-two outpatients with CF. Interventions Usual care (HA + HS followed by autogenic drainage) and combined therapy (HA + HS with oscillatory-PEP followed by autogenic drainage]. Each treatment was performed for 5 days. Main outcome measures Sputum expectoration was measured during the nebulisation period (primary outcome), during autogenic drainage and for 24 ho…

AdultMale030506 rehabilitationAdolescentCystic FibrosisPhysical Therapy Sports Therapy and RehabilitationCystic fibrosisPulmonary function testingPositive-Pressure RespirationYoung Adult03 medical and health sciences0302 clinical medicineAdministration InhalationmedicineHumansSingle-Blind Method030212 general & internal medicineHyaluronic AcidAdverse effectSaline Solution HypertonicCross-Over Studiesbusiness.industryNebulizers and VaporizersSputummedicine.diseaseCombined Modality TherapyCrossover studyConfidence intervalRespiratory Function TestsHypertonic salineClinical trialAnesthesiaSputumFemalemedicine.symptom0305 other medical sciencebusinessPhysiotherapy
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Early Osmotherapy in Severe Traumatic Brain Injury: An International Multicenter Study

2020

The optimal osmotic agent to treat intracranial hypertension in patients with severe traumatic brain injury (TBI) remains uncertain. We aimed to test whether the choice of mannitol or hypertonic saline (HTS) as early (first 96 h) osmotherapy in these patients might be associated with a difference in mortality. We retrospectively analyzed data from 2015 from 14 tertiary intensive care units (ICUs) in Australia, UK, and Europe treating severe TBI patients with intracranial pressure (ICP) monitoring and compared mortality in those who received mannitol only versus HTS only. We performed multi-variable analysis adjusting for site and illness severity (Injury Severity Score, extended IMPACT scor…

AdultMale030506 rehabilitationmedicine.medical_specialtyTraumatic brain injurymedicine.medical_treatmentosmotherapy03 medical and health sciences0302 clinical medicineOsmotherapyInternal medicineIntensive careDECOMPRESSIVE CRANIECTOMYBrain Injuries TraumaticHYPERTONIC SALINEmedicineMANAGEMENTHumansEQUIOSMOLARIntracranial pressureRetrospective StudiesSaline Solution Hypertonicbusiness.industryHead Traumatraumatic brain injuryHazard ratio3112 NeurosciencesmannitolMiddle Agedmedicine.disease3126 Surgery anesthesiology intensive care radiologyDiuretics Osmoticmortality3. Good healthHypertonic salineAdult Brain InjuryTreatment Outcome3121 General medicine internal medicine and other clinical medicineInjury Severity ScoreClinical Management of CNS InjuryDecompressive craniectomyFemaleNeurology (clinical)0305 other medical sciencebusinessCONSENSUS030217 neurology & neurosurgeryhypertonic saline
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